Journal: PLOS Pathogens

Article Title: Isolation and characterization of IgG3 glycan-targeting antibodies with exceptional cross-reactivity for diverse viral families

doi: 10.1371/journal.ppat.1012499

Figure Lengend Snippet: (A) HEp-2 cell slides were immunofluorescently stained with the indicated mAbs and goat anti-human Ig-FITC secondary (green) and DAPI (blue, to stain cell nuclei). Each mAb was tested at 100, 10, and 1 μg/mL. Control human serum samples that were positive or negative for anti-nuclear antibodies are shown (right). Images are 40X magnification. (B) Autoantigens were tested for reactivity by 2526, Ab82 (negative control antibody), and A32 (positive control antibody) using a commercially available AtheNA Multi-Lyte ANA kit. Values shown in bold were considered positive.

Article Snippet: Antibody reactivity to nine human autoantigens was measured using the AtheNA Multi-Lyte ANA kit (Zeus scientific, Inc, #A21101).

Techniques: Staining, Control, Immunopeptidomics, Negative Control, Positive Control

Journal: Neural Regeneration Research

Article Title: High mobility group box 1 mediates inflammatory response of astrocytes via cyclooxygenase 2/prostaglandin E2 signaling following spinal cord injury

doi: 10.4103/1673-5374.303039

Figure Lengend Snippet: Effects of TLR2/4 inhibitors on HMGB1-induced COX2 activation and PGE2 production in astrocytes. Primary cultured astrocytes were stimulated with 500 ng/mL rHMGB1 in the presence of 10 nM AO-I (TLR4 antagonist) or 10 nM C29 (TLR2 inhibitor) for 24 hours. The vehicle (0.1% dimethyl sulfoxide) was used as a control. (A) The COX2 protein level was determined by a western blot assay. (B) Quantification data of COX2 as shown in A. (C, D) PGE2 production in the lysate (C) and supernatant (D) was determined by an enzyme-linked immunosorbent assay. Experiments were performed in triplicate. Data are expressed as the mean ± SEM. * P < 0.05 (one-way analysis of variance followed by Tukey’s pos hoc test). AO-I: Atractylenolide I; COX2: cyclooxygenase 2; HMGB1: high mobility group box 1; PGE2: prostaglandin E2; rHMGB1: recombinant high mobility group box 1; TLR: Toll-like receptor.

Article Snippet: The PGE2 concentration in cell supernatants and lysates was measured using a PGE2 high-sensitivity enzyme-linked immunosorbent assay (ELISA) kit (Arbor Assays, Ann Arbor, MI, USA) according to the manufacturer’s instructions.

Techniques: Activation Assay, Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay, Recombinant

Journal: Neural Regeneration Research

Article Title: High mobility group box 1 mediates inflammatory response of astrocytes via cyclooxygenase 2/prostaglandin E2 signaling following spinal cord injury

doi: 10.4103/1673-5374.303039

Figure Lengend Snippet: HMGB1 fails to promote TNF-α and IL-1β production in astrocytes. An enzyme-linked immunosorbent assay was used to determine TNF-α (A, B) and IL-1β (C, D) production in the supernatants and lysates of astrocytes following treatment with 0, 1, 10, 100, or 1000 ng/mL rHMGB1 for 24 hours. Experiments were performed in triplicate. Data are expressed as the mean ± SEM and analyzed by one-way analysis of variance followed by Tukey’s post hoc test. HMGB1: High mobility group box 1; IL-1β: interleukin-1β; rHMGB1: recombinant high mobility group box 1; TNF-α: tumor necrosis factor-α.

Article Snippet: The PGE2 concentration in cell supernatants and lysates was measured using a PGE2 high-sensitivity enzyme-linked immunosorbent assay (ELISA) kit (Arbor Assays, Ann Arbor, MI, USA) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant